WebJan 20, 2024 · Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Hyclone, USA) supplemented with 10% fetal bovine serum (Hyclone, USA). Small intestines and their contents, which tested positive by RT-PCR, were homogenized and made into 20% suspensions using DMEM and 100 U/mL penicillin-streptomycin … WebJan 31, 2024 · The cells were seeded in basal medium containing L-DMEM (Hyclone, USA) and 8% fetal bovine serum (FBS, BI, USA) and cultured at 37 °C with 5% CO 2. Medium was changed every other days and cells were passaged when the cell confluence was about 90%. Cells were digested with 0.25% pancreatin at 37 °C for 2 min.
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WebVSMCs were cultured in basal DMEM/F12 medium (HyClone, Logan, Utah, USA), HUVECs were grown in RPMI 1640 medium (HyClone, Logan, Utah, USA), and 293 T cells were maintained in DMEM/high-glucose medium (HyClone, Logan, Utah, USA). The complete culture medium was supplemented with 10% fetal bovine serum (HyClone, Logan, Utah, … WebMice were injected intraperitoneally with 2 mL of 3.5% sterile thioglycollate solution (BD, Sparks, MD, USA); 4 days later, mice were sacrificed by cervical dislocation, and cells were isolated by peritoneal lavage with cold DMEM (Hyclone, Logan, UT, USA). how is twister played
HyClone™ Classical Liquid Media, DMEM F12, HyClone …
WebCulture your cells with Gibco DMEM F12. Gibco Dulbecco's Modified Eagle Medium: Nutrient Mixture F12 (DMEM F12) is a widely used basal medium for supporting the growth of many different mammalian cells including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. A 1:1 mixture of DMEM and Ham's F12 media, this ... WebOct 26, 2024 · After that, fibroblasts could be collected, then seeded to culture dishes, and allowed to adhere and proliferate in a humidified incubator with 5% CO 2 at 37 °C (Thermo Scientific, USA). DMEM was supplemented with 10% fetal bovine serum (FBS, Hyclone, UT, USA) and antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin, Gibco, USA). WebHeLa cells (from L. Johannes, Institute Curie, Paris, France) were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s high glucose medium (DMEM, HyClone, GE Healthcare Life Science, South Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS, Life Technology, Carlsbad, CA, USA), and 1% penicillin-streptomycin. how is twilight anesthesia administered