Solvent content of protein crystals
WebJan 4, 1995 · 2. Solvent Interactions with Proteins Revealed by X-Ray Crystallographic Studies . Edward N. Baker. Introduction. Solvent Content of Protein Crystals. Crystallographic Location of Solvent. The Crystallographic Location of Solvent. The Crystallographic Method. Identification and Refinement of Solvent Sites. Chemical … WebSolvent content of protein crystals. Matthews BW. Journal of Molecular Biology , 01 Apr 1968, 33(2): 491-497 DOI: 10. ... Molecular interactions in protein crystals: solvent accessible surface and stability. Islam SA, Weaver DL. Proteins, 8(1):1-5, 01 Jan 1990
Solvent content of protein crystals
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WebSolvent content of protein crystals. Solvent content of protein crystals. Solvent content of protein crystals J Mol Biol. 1968 Apr 28;33(2):491-7. doi: 10.1016/0022-2836(68)90205-2. … WebThis results in a water content insufficient to maintain the hydration of the biopolymers (or for complete protection of the biopolymers from one another). Salts, organic solvents and polymers are used as precipitants. Salts. A rather limited set of salts has been used to produce protein and nucleic acid crystals.
WebApr 28, 2016 · If by dehydration, one can lower their global solvent content maintaining the protein molecules order (Fig. 6.1b) (higher protein to solvent ratio) could lead to an improved diffracting power. Changes in solvent content need to be accompanied by small local rearrangements in the crystal contacts and/or flexible areas, therefore diffraction … WebMar 21, 2012 · The solvent content of the crystals was reduced by 3–6% by dehydration. This process also produces a change in their relative humidity from 99.2% to 98.5%. The application of molecular replacement, as detailed in the Experimental Section, enabled the identification of orientation and position of the two molecules in the asymmetric unit that …
WebFeb 13, 2024 · 2.2.2. Crystallization. For 20 samples, 380 mg lyophilized zinc-free insulin was dissolved in 20 ml double-distilled water (ddH 2 O). 2.31 ml 0.01 M zinc acetate solution was added to form insulin hexamers and the solution was aspirated lightly until no clouding was observed. After 5 min, 0.299 ml 1 M sodium thiocyanate solution was added to the … WebNov 17, 2003 · DOI: 10.1016/j.physa.2004.11.005 Corpus ID: 16651815; Solvent content of protein crystals from diffraction intensities by Independent Component Analysis …
WebProtein crystallization is the process of formation of a regular array of individual protein molecules stabilized by crystal contacts. If the crystal is sufficiently ordered, it will diffract.Some proteins naturally form …
WebOct 26, 2024 · By analyzing nearly 10,000 protein structures solved at high resolution, it is suggested that the number of detectable hydration water molecules depends on the resolution of structure analysis, the crystallographic R-factor, solvent content of the crystal, positional fluctuation of protein atoms reflected in the B-factors, percentage of amino ... flooring stores greeley coWebApr 25, 2012 · Protein crystals have catalytic and materials applications and are central to efforts in structural biology and therapeutic development. Designing predetermined crystal structures can be subtle given the complexity of proteins and the noncovalent interactions … great open world games pcWebNov 19, 2024 · Ice formation within protein crystals is a major obstacle to the cryocrystallographic study of protein structure, and has limited studies of how the structural ensemble of a protein evolves with temperature in the biophysically interesting range from ∼260 K to the protein–solvent glass transition near 200 K. Using protein crystals with … flooring stores fort worthWebFeb 13, 2016 · Artificial protein crystals have been designed by introducing and modifying functional moieties at the interface of proteins, such as fusion of two protein domains, which naturally form oligomeric proteins for the formation of novel protein crystals as reported in our recent review. 5 Co-crystallization of two different proteins provides … great operasWebAnother assumption is based on the nature of protein crystals, that is, protein crystals retain a relatively high solvent content compared to small molecule crystals. 37 Therefore, as … flooring stores houghton lakeWebMatthews BW. 1968. Solvent content of protein crystals. J Mol Biol 33:491-497. Kantardjieff KA, Rupp B. Matthews coefficient probabilities: Improved estimates for unit cell contents of proteins, DNA, and protein-nucleic acid complex crystals. Protein Science 12:1865-1871 … great operatic tenorsWebJan 2, 2009 · A nonredundant set of 9081 protein crystal structures in the Protein Data Bank was used to examine the solvent content, the number of polypeptide chains, and the oligomeric states of proteins in crystals as a function of crystal symmetry (as classified by crystal systems and space groups). flooring stores green bay wi